COURSE DESCRIPTION
This intensive course of one week is organised by the Erasmus Optical Imaging Centre. It gives an overview of the rapidly developing advanced fluorescence imaging methods and how they are used in biomedical research. An important part is the practical work each afternoon in which you get the opportunity for real hands-on experience. The focus of the practicals is on functional imaging (time lapse, FRAP, FRET) and super resolution (SIM, STED and single molecule tracking).
LEARNING GOALS
To get insight in and hands-on experience with advanced fluorescence imaging methods and data analysis
CONTENTS
The following topics will be covered during the course:
Lectures
Hands-on Practical Work
- Confocal Microscopy & Live Cell Imaging
- FRET, FRAP, TIRF, FLIM
- Super Resolution (SIM, STED, PALM/STORM, Rescan)
- Deconvolution
- Fluorescent Proteins
- Biological Applications
- Image Analysis
- Introduction to Confocal Microscopy
- Time Lapse Imaging
- FRAP
- Super Resolution Imaging (SIM, STED, PALM/STORM/Localisation Mic., Rescan, lightsheet)
- Data Analysis
- Data Presentation
FORMAT
This five day course consists of lecture sessions in the morning and practical sessions in the afternoon. On Monday and Thursday there are also evening sessions with dinner. In the final session on Friday afternoon participants present and discuss the results of one of the topics during the practical work.
REQUIRED BACKGROUND LEVEL
No prior knowledge is required
DURATION
50 hrs / 1.8 ECT
ORGANISERS
Erasmus Optical Imaging Centre (OIC)
Dr. Gert-Jan Kremers
DATES
6-10 November 2023
2024 TBA
LOCATION
Erasmus MC Rotterdam
MORE INFO
Click here for more information at the Erasmus' OIC website
REGISTRATION
Priority will be given to people that were registered for the cancelled 2021 edition of the course.
There are only limited spots available for ONWAR PhDs in this course. We will select applicants based on a short (max 200 words) motivation letter/e-mail, stating why you would like to follow this course (e.g. how it relates to your current work or future interests). To register, please send your motivation to registration@onwar.nl,